Kinetics of SV metabolite formation in human liver microsomes
| Subject | 3′-Hydroxy SV | 6′-Exomethylene SV | 3′,5′-Dihydrodiol SV | Total CLint ml/min/mg | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Km μM | Vmax pmol/min/mg | CLint ml/min/mg | Km μM | Vmax pmol/min/mg | CLint ml/min/mg | Km μM | Vmax pmol/min/mg | CLint ml/min/mg | ||
| 1 | 31.6 | 3083.9 | 0.10 | 59.4 | 1851.6 | 0.03 | 39.9 | 2639.6 | 0.07 | 0.20 |
| 2 | 12.8 | 1128.9 | 0.09 | 29.6 | 757.5 | 0.03 | 33.7 | 1416.3 | 0.04 | 0.16 |
| 3 | 19.7 | 2027.0 | 0.10 | 28.6 | 1298.8 | 0.05 | 27.6 | 2041.2 | 0.07 | 0.22 |
| 4 | 19.6 | 2024.5 | 0.10 | 27.0 | 1265.8 | 0.05 | 38.9 | 4047.1 | 0.10 | 0.25 |
| Mean | 20.9 | 2066.1 | 0.098 | 36.2 | 1293.4 | 0.037 | 35.0 | 2536.1 | 0.072 | 0.207 |
| SD | 7.8 | 799.5 | 0.007 | 15.5 | 447.1 | 0.011 | 5.6 | 1124.4 | 0.026 | 0.042 |
Values are means from duplicate determinations at each substrate concentration.