Kinetics of 5-HOS formation by human liver microsomes and microsomes derived from CYP c-DNA transfected B-lymphoblastoid cells
| Microsomes | Km | Vmax | Vmax/Km | Vmax* | Vmax*/Km |
|---|---|---|---|---|---|
| EFE556 | 17.8 | 404.8 | 22.8 | 1613.5 | 90.6 |
| FGL852 | 14.1 | 813.3 | 57.7 | 1982.9 | 140.6 |
| HGD057 | 18.3 | 948.1 | 51.8 | 2215.0 | 121.0 |
| FAG771 | 11.8 | 177.6 | 15.1 | 1119.5 | 94.9 |
| Mean | 15.5 | 589.0 | 36.9 | 1732.7 | 111.8 |
| SD | 3.1 | 357.0 | 21.0 | 478.0 | 23.5 |
| CYP2C8 | 32.9 | — | — | 498.3 | 15.2 |
| CYP2C9-arg | 16.5 | — | — | 410.6 | 24.9 |
| CYP2C9-cys | 47.4 | — | — | 163.5 | 3.5 |
| CYP2C19 | 63.7 | — | — | 190.5 | 3.0 |
| CYP3A4 | 311.7 | — | — | 264.1 | 0.9 |
Km , μM; Vmax, pmol of 5-HOS formed/mg protein/min;Vmax/Km , μl/mg protein/min; Vmax*, pmol of 5-HOS formed/nmol CYP/min; Vmax*/Km , μl/nmol CYP/min.