Effect of caffeine and 7,8-benzoflavone on cumene hydroperoxide (CHP)-dependent APAP-3-GSH formation
| (nmol APAP-3-GSH formed/min/mg protein) | |||
| Caffeine | 0 mM | 5 mM | % Activation |
| NADPH | 0.19 ± 0.01 | 0.92 ± 0.063-a | 373 |
| CHP | 0.45 ± 0.02 | 0.42 ± 0.02 | −5 |
| 7,8-Benzoflavone | 0 μM | 50 μM | % Activation |
| NADPH | 0.31 ± 0.02 | 1.15 ± 0.023-a | 273 |
| CHP | 0.40 ± 0.01 | 0.39 ± 0.02 | 0 |
Values are mean ± SD, N = 3
↵3-a No activator vs. + activator, p<0.001.