Table 1
Partitioning of propranolol, imipramine, and warfarin between serum and microsomes
| Propranolol | Imipramine | Warfarin | |
|---|---|---|---|
| fu(serum) | 0.12 ± 0.00 | 0.06 ± 0.00 | 0.0080 ± 0.0003 |
| fu(microsomes) 1-a | 0.38 ± 0.00 | 0.16 ± 0.01 | 0.85 ± 0.07 |
(calculated)1-b
| 0.32 | 0.38 | 0.0094 |
| 0.26 ± 0.01 | 0.31 ± 0.02 | 0.033 ± 0.002 |
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↵1-a Data from fig. 1.
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↵1-b Calculated from independently determined serum protein and microsome binding values in the first two rows.
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1-c Measured by dialysis of serum vs. microsomes.
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[14C]Warfarin (at 10 μM), [3H]imipramine (at 1.0 μM), and [3H]propranolol (at 1.0 μM) were dialyzedvs. human serum as well as in an experiment where serum and microsomes (at 1.0 mg/ml) were on opposite sides of the membrane. Values represent the mean ± SD of triplicate determinations.