Table 1

Apparent kinetic parameters describing the N-demethylation and 14-(R)-hydroxylation of CLAR in the presence of native human liver microsomes

Parameter1-aSubject Identification1-bMean ± SD1-c
HFOIENICF
Hydroxylation
KM 68.4  ± 6.034.1  ± 9.843.7  ± 9.548.7  ± 17.7
V max 293  ± 12151  ± 15173  ± 14206  ± 76
V max/KM 4.34.44.04.2  ± 0.21
N-Demethylation
KM 60.6  ± 10.234.3  ± 17.382.3  ± 22.159.1  ± 24
V max 209  ± 15130  ± 23227  ± 30189  ± 52
V max/KM 3.43.82.83.3  ± 0.53

  • ↵1-a Apparent KM (μM) and Vmax (pmol/min/mg protein) were determined using PCNONLIN. Intrinsic clearance (Vmax/KM ) was defined as μl/min/mg protein.

  • ↵1-b Data represent mean ± SE of the parameter estimate.

  • ↵1-c Mean ± SD of three subjects.