NADPH-dependent formation of methyl p-tolyl sulfoxide by human FMO3 expressed in insect cells, in E. coli, and by detergent-solubilized human liver microsomes
| Enzyme Source | Rate of Sulfoxide Formation | Stereochemistry |
|---|---|---|
| nmol/mg/min | % (R)-sulfoxide | |
| Human liver microsomes | 2.8 ± 1.4 | 54 ± 6 |
| (N = 4) | ||
| E. colimembranes | 24 ± 2.3 | 57 ± 1 |
| (N = 3) | ||
| T. ni membranes | 66 ± 20 | 56 ± 1 |
| (N = 5) |
Incubations were performed with 1 mM methyl p-tolyl sulfide at pH 8.5 in the presence of 0.5 mM NADPH. Data are expressed as the means ± SD.