Table 3

Effects of aldehyde oxidase and xanthine oxidase inhibitors on the oxidation of 50 μM 6-deoxypenciclovir and famciclovir with partially purified human, guinea pig, rabbit, and AO-active rat liver molybdenum hydroxylases monitored at 420 nm 3-a

Inhibitor (Conc.)Oxidation Rate (nmol/mg protein/min) of
6-DeoxypenciclovirFamciclovir
HumanGuinea PigRabbitAO-Active RatGuinea pigRabbitAO-Active Rat
Control2.3  ± 1.020.4  ± 5.630.0  ± 4.84.3  ± 1.759.3  ± 14.747.3  ± 5.415.0  ± 3.5
Menadione (1 μM)0.8  ± 0.23-c 3.6  ± 2.13-c 5.5  ± 3.13-b 2.5  ± 0.53-b 9.7  ± 8.33-c 5.1  ± 2.73-b 3.3  ± 1.23-c
Isovanillin (10 μM)0.6  ± 0.13-d 4.0  ± 2.23-d ND1.2  ± 0.63-c 10.0  ± 7.93-d ND3.0  ± 1.53-b
Allopurinol (100 μM)2.1  ± 0.119.0  ± 5.124.3  ± 3.03.6  ± 0.93-f 52.3  ± 14.640.7  ± 11.514.0  ± 3.5
  • 3-a Mean ± SD (N = 3).

  • Results are significantly different from control incubation at

  • 3-b p < 0.005,

  • 3-c p <0.02 and

  • 3-d p < 0.05 (pairedt-test).

    Not determined.

  • 3-f Oxidation rate of 6-deoxypenciclovir with AO-active rat liver enzyme in the presence of 100 μM menadione and 100 μM allopurinol = 0 nmol/mg protein/min.