Kinetic parameters for 6-oxo-metabolite formation by partially purified guinea pig, human, rabbit, rat liver molybdenum hydroxylases and bovine milk xanthine oxidase determined by HPLC
| Enzyme Fraction | 6-Deoxypenciclovir | Famciclovir | ||||
|---|---|---|---|---|---|---|
| KM (mM) | Vmax (nmol/mg/min) | Vmax/KM (ml/min/mg) | KM (mM) | Vmax (nmol/mg/min) | Vmax/KM (ml/min/mg) | |
| Human liver aldehyde oxidase (N = 1–2) | 0.42 | 16 | 0.038 | 0.15 | 61 | 0.407 |
| Guinea pig liver aldehyde oxidase (N = 3–5) | 0.41 ± 0.015-a | 209 ± 38 | 0.510 | 0.17 ± 0.01 | 439 ± 48 | 2.582 |
| Rabbit liver aldehyde oxidase (N = 2) | 0.44 | 114 | 0.259 | ND5-b | ||
| AO-Active rat enzyme (N = 3)5-a | 0.37 ± 0.05 | 26 ± 6 | 0.070 | 0.08 ± 0.02 | 41 ± 10 | 0.506 |
| AO-Deficient rat enzyme (N = 3)5-a | 0.26 ± 0.01 | 4 ± 2 | 0.015 | NR5-c | ||
| Bovine milk xanthine oxidase (N = 2) | 0.90 | 155 | 0.172 | NR | ||