Table 2

Apparent kinetic constants for BMO hydration to BDD by human, rat, and mouse liver microsomes^2-a

Species	V_max	K_m	V_max/K_m
	nmol/mg protein/min	mM
Human (H10)	55.8 ± 25.1^2-d	2.4 ± 0.8^2-c ^, ^2-e	23.2 ± 8.3^2-c
Human (H11)	26.7 ± 15.9	0.3 ± 0.2^2-b ^, ^2-e	95.6 ± 11.3^2-b ^, ^2-d ^, ^2-e
Mouse	4.0 ± 0.9^2-e	0.5 ± 0.4^2-b	12.4 ± 8.9^2-c ^, ^2-e
Rat	24.4 ± 1.6^2-d	0.7 ± 0.1^2-b	34.4 ± 6.1^2-c ^, ^2-d

↵2-a Human liver microsomal samples from H10, H11, or rat and mouse liver microsomes were incubated with BMO (0.1–10 mM, depending on the sample) in phosphate buffer for 15 min. The apparent kinetic constants were determined using a curve-fitting computer model as described in Materials and Methods. Values shown are means ± SD of the results obtained with 3–4 experiments.
↵2-b Significantly different from the corresponding H10 value.
↵2-c Significantly different from the corresponding H11 value.
↵2-d Significantly different from the corresponding mouse value.
↵2-e Significantly different from the corresponding rat value.