Table 1

Michaelis-Menten parameters for the primary pathways of DZ metabolism in hepatic microsomes and isolated hepatocytes from DEX-treated, PB-treated, and UT rats

TreatmentPathwayMicrosomes1-aHepatocytes1-b
VmaxKM CLintVmaxKM CLint
nmol/min μM μl/min nmol/min μM μl/min
DEX4′-HDZ0.23  ± 0.0711  ± 826.0  ± 10.40.19  ± 0.037  ± 333.4  ± 19.1
3-HDZ14.68  ± 1.70** 39  ± 9* 395.0  ± 136.3** 3.45  ± 0.72** 23  ± 3** 157.5  ± 49.2**
NDZ1.26  ± 0.22* 23  ± 6* 58.8  ± 25.2* 0.33  ± 0.06* 14  ± 4** 24.8  ± 8.0**
PB 4′-HDZ0.13  ± 0.047  ± 222.9  ± 17.80.17  ± 0.049  ± 3* 20.4  ± 4.3**
3-HDZ7.81  ± 1.18** 135  ± 2758.5  ± 3.2** 4.67  ± 0.86** 65  ± 19 73.2  ± 10.5**
NDZ2.70  ± 0.73** 54  ± 9* 50.7  ± 14.9** 1.48  ± 0.19** 56  ± 13* 26.7  ± 2.7**
UT1-c4′-HDZ0.16  ± 0.075  ± 239.9  ± 27.60.20  ± 0.053  ± 157.9  ± 9.1
3-HDZ2.29  ± 0.64119  ± 3219.1  ± 0.81.19  ± 0.2171  ± 11 17.3  ± 4.5
NDZ0.78  ± 0.1535  ± 522.3  ± 1.90.25  ± 0.0235  ± 57.1  ± 1.1
  • Data are presented as means ± SD (N = 4). Mean from induced states significantly different from UT (* p < 0.05, ** p < 0.01). Hepatocyte KM significantly different from microsomal KM (

  • dagger; p < 0.05).

  • 1-a Vmax andCLint are expressed per milligram of microsomal protein.

  • 1-b Vmax andCLint are expressed per million cells.

  • 1-c Data from Zomorodi et al. (9).