Table 1

Concentration of ISIS 2922 and total radioactivity in vitreous and retina after a single intravitreal injection

Time Post-Injection (hr)	Vitreous		Retina
Time Post-Injection (hr)	μM-Equivalent	Fraction Coeluting on HPLC with Intact	ISIS 2922 (μM)	μM-Equivalent	Fraction Coeluting on HPLC with Intact	ISIS 2922 (μM)
4	5.61	0.59	3.31	3.0	0.48	1.46
24^1-i	4.04 ± 0.17	0.47 ± 0.03	1.89 ± 0.19	5.37 ± 0.22	0.42 ± 0.03	2.24 ± 0.16
72	3.71	0.34	1.28	9.23	0.29	2.79
120	2.96	0.40	0.75	9.43	0.31	3.51
168	0.90	0.27	0.24	2.7	0.15	0.41
240	0.75 ± 0.065	0.22 ± 0.16	0.17 ± 0.13	6.5 ± 0.62	0.24 ± 0.03	1.56 ± 0.27
288	0.42	0.29	0.12	1.65	0.14	0.14
336	0.31	0.03	0.01	1.98	0.23	0.46
384	0.17	0.13	0.02	2.62	0.17	0.25
432	0.22 ± 0.045	0.18	0.04 ± 0.03	2.90 ± 1.22	0.18 ± 0.04	0.54 ± 0.28
480	0.18	0.08	0.02	1.22	0.09	0.11

↵1-i SDs are shown for the three time points, 24, 240, and 432 hr, which had three animals.
The concentration of [¹⁴C] derived from [¹⁴C]-radiolabeled ISIS 2922, the percent of the radiolabel coeluting with intact ISIS 2922 on HPLC, and the concentration derived by multiplying those two parameters, are shown over time after a single intravitreal injection of 66 μg of [¹⁴C]-ISIS 2922.