Kinetics of glucuronidation of R- and S-propranolol and androsterone using membrane preparations of HK293 cells stably expressing UGT2B7Y or H
| Substrate | UGT2B7Y | UGT2B7H | ||||
|---|---|---|---|---|---|---|
| Km (μM) | Vmax pmol/min/mg protein | Efficiency μl/min/mg (×100) | Km(μM) | Vmax pmol/min/mg protein | Efficiency μl/min/mg (×100) | |
| S-propranolol | 184, 54 | 70, 140 | 38, 259 | 72 ± 19 | 148 ± 30 | 205 |
| R-propranolol | 180 | 96 | 53 | 101, 72 | 194, 86 | 192 |
| Androsterone | 7.4, 6.1 | 372, 962 | 5027, 15874 | 7.2, 12.2 | 584, 909 | 8100, 7443 |
The assays were performed as described in the Methodssection.