Inhibition of rat hepatic microsomal mono-oxygenase activity by SKF-525A with or without preincubation prior to substrate addition2-a
| Reaction | Inhibitor | No Preincubation | Preincubation (5 min) | ||
|---|---|---|---|---|---|
| KM (mM) | Vmax (nmol/ml/mg protein) | KM (mM) | Vmax (nmol/ml/mg protein) | ||
| Aminopyrine | None | 1.25 | 4.0 | 1.9 | 2.8 |
| N-demethylation | SKF-525A (50 μM) | 7.1 | 4.0 | 1.9 | 1.4 |
| Aniline | None | 0.09 | 0.67 | 0.16 | 0.73 |
| Para-hydroxylation | SKF-525A (50 μM) | 0.25 | 0.67 | 0.16 | 0.33 |
| Ethylmorphine | None | 0.30 | 20 | 0.26 | 18 |
| N-demethylation | SKF-525A (50 μM) | 0.72 | 17 | 0.31 | 4 |
↵2-a Adapted from Franklin, 1977.