Table 1

Identified binding sites for covalent binding of BG to HSA (in the presence of NaCNBH3)

HPLC Peak1-aHSA ResiduesMolecular Weights of Peptides1-bSequence InformationModifications1-c
1191–1971208.5, 1190.5PSD, CIDBG-K-1951-d
2187–1951353.5, 1335.5PSD, CIDBG-K-190
3517–5211109.4PSDBG-K-519
440–4451198.4PSD, CIDBG-K-444
4411–4141000.3, 982.3PSD, CIDBG-K-413
5433–4391301.5, 1283.5PSDBG-K-436
182–1951979.8, 1961.8PSD, CIDBG-K-190
6539–5451278.5, 1260.5PSD, CIDBG-K-5411-d
7157–160982.3, 964.3PSD, CIDBG-K-159
8157–160982.3, 964.3PSD, CIDBG-K-159
198–2051409.6PSD, CIDBG-K-1991-d
9461–4721902.8PSDBG-K-466
10205–2091097.4PSD, CIDBG-K-205
11508–5212168.8, 2150.8PSD, CIDBG-K-519
12156–1601129.4, 1111.4PSDBG-K-159
1371–811695.7PSDBG-K-73
14210–2181480.6, 1462.6PSD, CIDBG-K-212
1565–812394.1PSD, CIDBG-K-73
16145–1602516.2PSDBG-K-159
17146–1602360.1PSD, CIDBG-K-159

  • ↵1-a HPLC peak numbers correspond to the labeled peaks in fig. 1.

  • ↵1-b Lower molecular weights (−18 mass units) correspond to water-loss ions generated by MALDI.

  • ↵1-c K, lysine.

  • ↵1-d Modification sites also found for TG.