Michaelis-Menten kinetic parameters in human recombinant isozymes2-a
| Isozyme | Km [AE] (μM) | Vmax (nmol DQHS/min/nmol P450) | Clint (L/min/nmol P450) |
|---|---|---|---|
| CYP2B6 | 28.1 ± 5.0 | 1.36 ± 0.08 | 0.05 |
| CYP3A4 | 42.0 ± 3.3 | 12.64 ± 1.05 | 0.30 |
| CYP3A5 | 24.9 ± 6.3 | 2.75 ± 0.38 | 0.11 |
↵2-a Arteether at various concentrations was incubated with recombinant isozymes of human cytochrome P450. DQHS production was measured and the Michaelis-Menten parameters were calculated as described in Materials and Methods (mean ± SD,N = 4).