Table 1

Estimated kinetic parameters for the formation of nirvanol from nirvanol-free (S)-MP (0.5–200 μM), in the presence or absence of sulfaphenazole (25 μM), in pooled HLMs (HL2, HL8, and HL9)

Incubation with	Kinetics of Nirvanol Formation
Incubation with	K_M,₁	V_max,1	V_max,/K_M,₁	K_M,₂	V_max,2	V_max,2/K_M,₂
	μM	pmol/min/mg protein		μM	pmol/min/mg protein
(S)-MP alone	174.1	170.5	0.98	1,910	3,980	2.1
(S)-MP + sulfaphenazole				2,706	22,690	8.4

K_M,₁ and V_max,1refer to Michaelis-Menten parameters for the high-affinity component, whereas K_M,₂ and V_max,2 refer to the low-affinity component. Values are means of estimates from nonlinear least-squares regression analysis, performed using WINNONLIN (seeMaterials and Methods).