Table 1

Rates of quinone-type metabolite 3 formation from troglitazone catalyzed by recombinant CYP3A4 in the presence of an NADPH-generating system or CuOOH

CYP3A4Metabolite 3 Formation
NADPH-generating systemCuOOH
nmol product/min/nmol CYP3A4
Purified CYP3A42.86.6

Troglitazone (30 μM) was incubated at 37°C in the presence of 0.025 μM recombinant CYP3A4 and an NADPH-generating system (for 20 min) or 0.1 mM CuOOH (for 5 min) as described in Materials and Methods. Results are represented as means of duplicate determinations.