CYP3A4 | Metabolite 3 Formation | |
---|---|---|
NADPH-generating system | CuOOH | |
nmol product/min/nmol CYP3A4 | ||
Supersomes | 11 | 18 |
Purified CYP3A4 | 2.8 | 6.6 |
Troglitazone (30 μM) was incubated at 37°C in the presence of 0.025 μM recombinant CYP3A4 and an NADPH-generating system (for 20 min) or 0.1 mM CuOOH (for 5 min) as described in Materials and Methods. Results are represented as means of duplicate determinations.