Effects of preimmune and anti-P-450 antibodies on quinone-type metabolite 3 formation of troglitazone catalyzed by human liver microsomes (samples HL-3 and HL-4)
| Antibody | mg IgG/nmol P-450 | Metabolite 3 Formation | |
|---|---|---|---|
| HL-3 | HL-4 | ||
| pmol/min/nmol P-450 | |||
| None | 114 (100) | 123 (100) | |
| Preimmune | 20 | 90 (79) | 96 (75) |
| Anti-CYP2C | 10 | 73 (64) | 18 (14) |
| 20 | 63 (55) | 17 (13) | |
| Anti-CYP3A | 10 | 52 (46) | 76 (62) |
| 20 | 41 (36) | 68 (55) | |
Troglitazone (10 μM) was incubated with human liver microsomes (samples HL-3 and HL-4) in the absence or presence of preimmune, anti-rat CYP2C13, and anti-rat CYP3A2 antibodies. Data are means of duplicate determinations. Values in parenthesis indicate percentage of control (none).