Michaelis-Menten kinetic parameters of S-mephobarbital N-demethylation in cDNA-expressed CYP2B6 and human liver microsomes
| Microsomes | Vmax | Km | Vmax/Km |
|---|---|---|---|
| pmol/min/mg | μM | μl/min/mg | |
| CYP2B6 | 2.9 | 264 | 0.011 |
| HL34 | 5.4 | 236 | 0.023 |
| HL38 | 4.6 | 276 | 0.017 |
The concentration range of S-mephobarbital used was 100 μM to 1 mM. HL, human liver.