Metabolite | Hydroxylated Testosterone Metabolite Formed in | |||||||
---|---|---|---|---|---|---|---|---|
Freshly isolated cells | Cryopreserved cells | |||||||
t = 0 min | t = 30 min | t = 60 min | t = 120 min | t = 0 min | t = 30 min | t = 60 min | t = 120 min | |
pmol/min/mg protein | ||||||||
6β-OHT | 626 ± 157 | 517 ± 155 | 525 ± 57 | 335 ± 52* | 468 ± 124 | 442 ± 136 | 398 ± 71 | 405 ± 179 |
2β-OHT | 76 ± 18 | 62 ± 15 | 67 ± 11 | 39 ± 5* | 62 ± 19 | 54 ± 14 | 51 ± 12 | 47 ± 24 |
Data are expressed as mean ± S.D. of four independent human PC isolations and their corresponding cryopreserved counterparts.
∗, significantly different from the corresponding t = 0 value (p < .05, Dunnett's test).