Table 2

Formation of hydroxylated testosterone metabolites in freshly isolated and cryopreserved rat liver PC held in suspension at 37°C for up to 2 h

Metabolite	Hydroxylated Testosterone Metabolite Formed in
	Freshly isolated cells		Cryopreserved cells
	t = 0 min	t = 30 min	t = 60 min	t = 120 min	t = 0 min	t = 30 min	t = 60 min	t = 120 min
	pmol/min/mg protein
16α-OHT	582 ± 150	522 ± 108	530 ± 89	505 ± 103	557 ± 158	523 ± 133	478 ± 104	355 ± 97
2α-OHT	523 ± 166	498 ± 150	459 ± 125	430 ± 129	421 ± 126	352 ± 110	350 ± 94	238 ± 77
6β-OHT	226 ± 34	177 ± 25	167 ± 50	128 ± 27^*	180 ± 38	204 ± 36	135 ± 22	86 ± 18^*
2β-OHT	60 ± 15	42 ± 18	31 ± 7^*	17 ± 3^*	41 ± 16	31 ± 7	22 ± 6	14 ± 4^*
7α-OHT	57 ± 15	42 ± 10	40 ± 13	38 ± 11	36 ± 11	39 ± 10	31 ± 7	24 ± 7
15β-OHT	56 ± 12	55 ± 9	49 ± 8	50 ± 10	53 ± 7	55 ± 11	49 ± 8	41 ± 7

Data are expressed as mean ± S.D. of four independent rat PC isolations and their corresponding cryopreserved counterparts.

∗, significantly different from the corresponding t = 0 value (p < .05, Dunnett's test).