Table 1

Effect of solvents on cDNA-expressed human cytochromes P-450 activities in human lymphoblastoid cell microsomes

Enzyme	Solvent	% Solvent
Enzyme	Solvent	0.1%	0.3%	1%	3%	5%
		% Inhibition
CYP1A1	Methanol		8 ± 7	26 ± 7	70 ± 14
	Ethanol	19 ± 2	38 ± 12	69 ± 6	97 ± 5
	DMSO		6 ± 3	24 ± 7	57 ± 8
	Acetonitrile		26 ± 6	58 ± 6	87 ± 3	94 ± 2
CYP1A2	Methanol		0 ± 8	9 ± 11	12 ± 10
	Ethanol		2 ± 9	3 ± 5	30 ± 3
	DMSO		(−1) ± 9	0 ± 5	12 ± 4
	Acetonitrile		2 ± 1	1 ± 0	36 ± 4	71 ± 7
CYP2A6	Methanol		(−2) ± 5	(−3) ± 4	8 ± 6
	Ethanol		0 ± 7	18 ± 7	44 ± 4
	DMSO		2 ± 3	3 ± 4	8 ± 4
	Acetonitrile		3 ± 1	13 ± 1	37 ± 4	65 ± 3
CYP2B6	Methanol		5 ± 2	19 ± 2	42 ± 15
	Ethanol	25 ± 2	52 ± 3	80 ± 2	97 ± 2
	DMSO	6 ± 5	13 ± 4	27 ± 8	50 ± 7
	Acetonitrile		13 ± 2	42 ± 2	84 ± 2	96 ± 3
CYP2C8	Methanol		(−4) ± 5	(−8) ± 5	(−3) ± 6
	Ethanol		4 ± 3	(−2) ± 5	16 ± 10
	DMSO		2 ± 2	7 ± 4	10 ± 8
	Acetonitrile		1 ± 7	(−4) ± 6	19 ± 1	63 ± 6
CYP2C9	Methanol		1 ± 5	12 ± 5	13 ± 1
	Ethanol		6 ± 6	6 ± 6	7 ± 6
	DMSO		10 ± 3	12 ± 7	18 ± 3
	Acetonitrile		1 ± 8	(−12) ± 5	(−14) ± 14	(−8) ± 15
CYP2C19	Methanol		2 ± 5	9 ± 3	37 ± 7
	Ethanol	28 ± 5	53 ± 6	72 ± 3	91 ± 2
	DMSO	17 ± 8	30 ± 9	62 ± 2	88 ± 1
	Acetonitrile		2 ± 11	10 ± 4	43 ± 2	73 ± 4
CYP2D6	Methanol		7 ± 4	26 ± 2	57 ± 7
	Ethanol	11 ± 3	24 ± 6	59 ± 5	83 ± 2
	DMSO	16 ± 1	38 ± 7	67 ± 6	87 ± 3
	Acetonitrile		3 ± 8	19 ± 5	48 ± 10	70 ± 7
CYP3A4	Methanol		0 ± 8	4 ± 9	25 ± 5
	Ethanol		0 ± 10	3 ± 13	40 ± 7
	DMSO	23 ± 2	25 ± 9	43 ± 7	67 ± 4
	Acetonitrile		2 ± 1	11 ± 3	37 ± 6	65 ± 6

Values are expressed as the mean ± S.D. (N = ≥2).