Table 3

Uptake of E₁S in presence of 0, 10, 25, and 100 μM pregnenolone sulfate (in 1% ethanol) by isolated rat hepatocytes

	K_m	V_max	P_diff	K_i
	μM	nmol/min/mg protein	μl/min/mg protein	μM
Control (0% ethanol;n = 6)^3-a	26 ± 8.3	1.8 ± 0.26	3.2 ± 1.0
Control (1% ethanol; n = 4)^3-a	25 ± 9	1.8 ± 0.81	5.9 ± 1.6^3-150
Added PS (1% ethanol;n = 4)^3-b	37 ± 14	2.8 ± 0.72	2.7 ± 0.9	6.7 ± 0.9

↵3-a Best fitted to eq. 2; a weighting of 1/prediction was optimal.
↵3-b All data (0, 10, 25, and 100 μM pregenolone sulfate) were simultaneously fitted to eq. 6, and weighting scheme of 1/prediction was optimal.
↵3-150 Different from 0% ethanol controls (ANOVA, p < .05).