Apparent inhibition kinetics of ketoconazole toward EROD activity
| Enzyme Source | Km | Vmax | Clint | Ki |
|---|---|---|---|---|
| nM | pmol/min/mg | mL/min/mg | nM | |
| Microsomes | ||||
| HG-19 | 50.3 | 33.1 | 0.66 | 49.1 |
| HG-26 | 34.2 | 48.2 | 1.41 | 36.0 |
| HG-30 | 53.0 | 122.2 | 2.31 | 33.8 |
| Mean | 45.8 | 67.8 | 1.46 | 39.6 |
| (S.D.) | (10.2) | (47.7) | (0.83) | (8.3) |
| pmol/min/pmol | mL/min/pmol | |||
| Expressed CYP1A1 | 24.1 | 7.6 | 0.32 | 36.6 |