Summary of studies reporting expression and quantification of CYP2B6 protein in hepatic tissue
Incidence of Detection | pmol/mg | Antibody | Standard | Notes | Reference | |||
---|---|---|---|---|---|---|---|---|
Mean (S.D.) | High | Low | ||||||
% | ||||||||
2 /10 | 20 | —1-a | — | — | pAb anti-CYP2B1 | rCYP2B61-b (microsomal) | Baker et al., 1995 | |
6 /30 | 20 | — | — | — | pAb anti-CYP2B1 peptide | rCYP2B6 (microsomal) | Edwards et al., 1998 | |
8 /30 | 27 | — | — | — | pAb anti-CYP2B6 peptide | rCYP2B6 (microsomal) | Boobis et al., 1996 | |
9 /30 | 30 | ∼0.251-c | ∼21-c | 0 | pAb anti-monkey CYP2B17 | Monkey CYP2B17 | Japanese population | Shimada et al., 1994 |
26 /30 | 87 | 11-c | ∼2.51-c | 0 | pAb anti-monkey CYP2B17 | Monkey CYP2B17 | Caucasian population | Shimada et al., 1994 |
? /40 | — | 0.14 (0.62) | — | — | pAb anti-monkey CYP2B17 | Monkey CYP2B17 | Japanese population | Guengerich, 1995 |
? /32 | — | 1.4 (1.8) | — | — | pAb anti-monkey CYP2B17 | Monkey CYP2B17 | Caucasian population | Guengerich, 1995 |
12 /141-c | 86 | — | — | — | pAb anti-CYP2B1 | rCYP2B6 (lysate) | Thai population; “high level of CYP2B6 expression” | Kirby et al., 1993 |
17 /17 | 100 | 15 (21) | 74 | 0 | pAb anti-CYP2B1 | rCYP2B6 (microsomal) | Code et al., 1997 | |
? /16 | ∼31-c | ∼171-c | 0? | pAb anti-rCYP2B6 | Purified rCYP2B6 | Imaoka et al., 1996 | ||
2 /10 | 20 | 2.65 (5.94) | — | — | pAb anti-rCYP2B6 | Purified rCYP2B6 | Ages perinatal through 1 yr old | Tateishi et al., 1997 |
7 /10 | 70 | 19.36 (23.9) | — | — | pAb anti-rCYP2B6 | Purified rCYP2B6 | Tateishi et al., 1997 | |
24 /26 | 92 | 10.9 (9.5) | 28 | 0 | mAb anti-rCYP2B6 | Purified rCYP2B6 | Yang et al., 1998 | |
12 /121-c | 100 | — | — | — | pAb anti-CYP2B1 | rCYP2B6 (lysate) | CYP2B6 high in donor with phenobarbital medication | Forrester et al., 1992 |
12 /50 | 24 | ∼1.4 (1.5)1-c | 7 | 0 | pAb anti-monkey CYP2B17 | Monkey CYP2B17 | Mimura et al., 1993 | |
13 /131-c | 100 | — | — | — | pAb anti-CYP2B1 | CYP2B1 | CYP2B6 comigrated with CYP2B11-d | Yamano et al., 1989 |
19 /19 | 100 | 9.77 (17.6) | 71.1 | 0.7 | mAb anti-rCYP2B6 | rCYP2B6 (microsomal) | Ekins et al., 1998 | |
28 /28 | 100 | 24.9 (18.2) | 82 | 2 | pAb anti-CYP2B6 peptide | rCYP2B6 (microsomal) | Present study |