Induction of UGT (4-MUF as substrate) and EROD activities by treatment of Caco-2 cell clones TC7 and PF11 with TBHQ or TCDD
| Enzyme Treatment | Enzyme activity1-a | |
|---|---|---|
| Caco-2, clone TC7 | Caco-2, clone PF11 | |
| UGT | (nmol/min/mg protein) | |
| Controls | 7.7 ± 0.8 | 0.6 ± 0.1 |
| TBHQ (80 μM) | 15.8 ± 3.0 (2.1)1-b | 1.5 ± 0.3 (2.5) |
| TCDD (10 nM) | 12.6 ± 1.8 (1.6) | 1.9 ± 1.2 (3.2) |
| EROD | (pmol/min/mg protein) | |
| Controls | 3.9 ± 0.9 | 3.1 ± 0.9 |
| TBHQ (80 μM) | 13.1 ± 6.8 (3.4) | 7.2 ± 2.7 (2.3) |
| TCDD (10 nM) | 379 ± 146 (97) | 252 ± 123 (81.3) |