Selected biochemical parameters and activities of hepatic drug-metabolizing enzymes in CF-1 mice
| Parameter/Activity | Animal Group | |||
|---|---|---|---|---|
| Male (+/+) | Male (−/−) | Female (+/+) | Female (−/−) | |
| B.wt. at 4 weeks (g) | 27.1 | 28.2 | 23.1 | 23.7 |
| Liver weight (% of body weight) | 6.0 | 6.7 | 5.3* | 6.0 |
| Microsomal protein (mg/g liver) | 3.5 ± 0.76 | 2.79 ± 0.80 | 2.9 ± 1.67 | 3.7 ± 0.22 |
| Cytosolic protein (mg/g liver) | 27.5 ± 5.1 | 26.9 ± 4.0 | 25.3 ± 11.9 | 18.8 ± 3.8 |
| Total P-450 (nmol/mg) | 0.60 ± 0.10 | 0.51 ± 0.06 | 0.43 ± 0.12 | 0.35 ± 0.12 |
| CYP 3A1-a (pmol/mg) | 166.5 ± 5.9 | 173.0 ± 4.1 | 176.9 ± 11.7 | 174.4 ± 3.8 |
| CYP 2B1-b (pmol/mg) | 120.9 ± 3.5 | 122.8 ± 3.8 | 119.7 ± 4.9 | 119.5 ± 2.3 |
| CYP 3A1-c (O.D. 655 nm) | 0.023 ± 0.005 | 0.031 ± 0.008 | 0.028 ± 0.005 | 0.024 ± 0.003 |
| Testosterone 6β hydroxylation (nmol/min/mg) | 5.80 ± 1.80 | 5.58 ± 0.52 | 5.56 ± 1.11 | 6.96 ± 1.11 |
| Nifedipine oxidation (nmol/min/mg) | 3.77 ± 0.42 | 4.01 ± 0.39 | 3.62 ± 0.79* | 5.0 ± 0.4* |
| NADPH cyt c reductase (nmol/min/mg) | 99.1 ± 24.6 | 126.6 ± 15.5 | 127.5 ± 30.1* | 166.6 ± 12.5* |
| Glutathione S-transferase (μmol/min/mg) | 6.68 ± 1.14 | 6.21 ± 0.22 | 1.84 ± 0.74* | 3.69 ± 0.57* |
Results are means ± S.D. (n = 4, each sample consists of three pooled liver samples). Asterisk (*) denotes significant differences from (−/−) animals of the same sex group,p < .05.
↵1-a Determined by ELISA using anti-rat CYP 3A2 antibody as probe.
↵1-b Determined by ELISA using anti-rat CYP 2B1 antibody as probe.
↵1-c Determined by immunoblotting using anti-CYP 3A4 as probe.