Effect of different xanthates on the activities of P-450 2D6 and P-450 2C9
| Isozyme | Xanthate | Concentration | % Activity Remaining | |
|---|---|---|---|---|
| 0 min | 15 min | |||
| μM | ||||
| P450 2D6 | None | 100 | 89 | |
| C3iso | 40 | 93 | 85 | |
| 400 | 56 | 47 | ||
| D609 | 60 | 85 | 78 | |
| 600 | 79 | 63 | ||
| C8 | 20 | 93 | 76 | |
| 200 | 52 | 49 | ||
| C14 | 50 | 95 | 78 | |
| P450 2C9 | None | 100 | 87 | |
| C3iso | 400 | 123 | 95 | |
| 800 | 148 | 132 | ||
| D609 | 60 | 89 | 85 | |
| 600 | 79 | 79 | ||
| C8 | 20 | 93 | 83 | |
| 200 | 60 | 51 | ||
| C14 | 50 | 68 | 68 | |
Assay conditions were as described in Experimental Procedures. The 100% activities ranged from 36 to 40 pmol of product formed min−1 pmol−1 for P450 2C9 and from 60 to 80 pmol of product formed min−1 pmol−1 for P450 2D6. The data shown represent the mean of two different experiments.