Table 3

Effect of PSC 833 on the tissue-to-plasma concentration ratio of [3H]VCR

TissueDose of PSC 833
Control0.1 mg/kg0.3 mg/kg3 mg/kg
Brain1.07  ± 0.051.64  ± 0.041.34  ± 0.151.37  ± 0.19
Liver152  ± 21229  ± 26153  ± 270.3  ± 10.63-150
Intestine165  ± 26235  ± 33218  ± 17155  ± 19
Kidney168  ± 28258  ± 17180  ± 18175  ± 4
Adrenal203  ± 25277  ± 28159  ± 23125  ± 11
Spleen242  ± 33344  ± 21245  ± 27179  ± 5
Lung212  ± 22343  ± 44274  ± 50162  ± 33

[3H]VCR was administered i.v. to rats that had received an i.v. injection of PSC 833 (0, 0.1, 0.3, and 3 mg/kg) 30 min before the experiments. At 2 h after administration of [3H]VCR, the tissue-to-plasma concentration was determined. For the plasma, liver, and intestine, HPLC was used to determine the intact [3H]VCR. For other tissues, the total radioactivity was used because we found that more than 80% of the radioactivity was associated with intact [3H]VCR in these tissues. Results are given as the mean ± S.E. of three independent experiments.

  • 3-150P < .05, significantly different from control, using Fisher’s t test.