Table 3

Detection of CYP mRNAs and proteins in human small intestine

P-450 FormsmRNA (by RT-PCR)Protein3-a (by Immunoblot)
1A1+ (weak)+
1A2
1B1+
2A6
2A7
2B6
2C+++
2D6+
2E1+ (weak)
2F1
3A4++++
3A5+
3A7
4B1

CYP mRNA expression was detected by RT-PCR as described inExperimental Procedures. Each primer pair was designed to specifically detect a single CYP form except the CYP2C primer pair, which detects all forms of the CYP2C subfamily. As a positive control, all CYPs were detected in RNA samples from human liver analyzed with the same primers and under identical conditions except for CYP2A7, 2F1, and 4B1 (not shown). CYP protein expression was analyzed by immunoblotting analysis as described in the legend to Fig. 2.

  • +, positive detection of DNA fragments of predicted size or CYP immunoreactive band; −, product of predicted size or an immunoreactive band were not detected.

  • 3-a +, weak intensity; ++, intermediate intensity; +++, strong intensity.