Table 2

Estimates of K_i (μM) obtained from three methods of data analysis applied to one set of parameters^2-a

Data Set ^2-b	SNLR: V_MAX andK_M		K_M,app:V_MAX		Dixon [S]
Data Set ^2-b	Fixed	Varying	Fixed	Varying	10	25	100	250	500
[I] = 0–100 (6)			unwtd (wt = 1/y)		[S] = 10–1000 (6)^2-c
1	4.71	4.69	5.03	5.06	4.30	8.06	5.31	21.14	−1.94
2	4.60	5.24	4.54	4.31	1.93	4.82	1.05	−9.73	497.00
3	4.92	4.91	4.58	4.96	6.60	3.21	2.29	−5.49	57.93
Mean	4.74	4.95	4.72 (4.74)	4.78 (5.09)	4.28	5.37	2.88	1.97	184.33
S.D.	0.16	0.27	0.27 (0.25)	0.41 (0.23)	2.34	2.47	2.19	16.74	272.43

[I] = 0–75 (5)
1	4.66	4.67	4.07	3.66
2	5.43	5.28	4.60	5.18
3	4.90	4.90	3.87	3.94
Mean	5.00	4.95	4.18 (4.65)	4.26 (5.05)
S.D.	0.40	0.31	0.38 (0.16)	0.81 (0.54)

[I] = 0–50 (4)
1	4.77	4.82	5.39	5.43
2	5.55	5.46	5.69	6.38
3	5.05	5.07	4.98	5.07
Mean	5.12	5.12	5.35 (4.97)	5.62 (5.49)
S.D.	0.39	0.32	0.36 (0.29)	0.68 (0.47)

[I] = 0–25 (3)
1	4.79	4.86	6.56	6.45
2	5.27	5.20	3.91	5.53
3	5.14	5.19	6.05	6.48
Mean	5.06	5.08	5.50 (4.92)	6.15 (5.56)
S.D.	0.25	0.19	1.41 (0.80)	0.54 (0.17)

[I] = 0–10 (2)
1	3.91	3.90
2	5.61	5.64
3	4.60	4.60
Mean	4.71	4.71
S.D.	0.85	0.88

↵2-a Perfect simulated data were generated with following values: V_MAX = 100 nmol/min · mg protein;K_M = 10 μM; K_i = 5.0 μM.
↵2-b Range and number (in parenthesis) of inhibitor ([I]) concentrations used, including a control.
↵2-c Range and number (in parenthesis) of substrate ([S]) concentrations used.