Michaelis-Menten kinetic parameters of POD in microsomes from baculovirus-infected insect cells expressing CYP1A1, CYP1A2, or CYP2E1
| CYP1-a | Km | Vmax |
|---|---|---|
| μM | pmol/min/pmol CYP | |
| CYP1A1 | 27 ± 3 | 47 ± 0.6 |
| CYP1A2 | 28 ± 2 | 20 ± 0.4 |
| CYP2E1 | 785 ± 125 | 4.3 ± 0.4 |
↵1-a Eight pmol/ml CYP1A1 or CYP1A2 and 20 pmol/ml CYP2E1 were incubated for 30 min, respectively. The concentration range of phenacetin used was 1 μM to 1 mM.