Table 1

Experimental conditions for the five probe assays using different enzyme sources

SubstrateIsoformExpression SystemIncubation TimeProtein Concentration
min mg/ml (pmol CYP/ml)
E. coli membranes150.1  (22)
EthoxyresorufinCYP1A2B-lymphoblasts150.1  (14)
HLM150.1
E. colimembranes150.2  (77)
NaproxenCYP2C9B-lymphoblasts51.4  (67)
HLM51
E. colimembranes1-a 150.7  (40)
Baculosomes150.4  (40)
DiazepamCYP2C19B-lymphoblasts150.2  (50)
HLM200.25
E. colimembranes150.06  (32)
DextromethorphanCYP2D6B-lymphoblasts150.24  (8.4)
HLM150.85
E. colimembranes100.05  (25)
ErythromycinCYP3A4B-lymphoblasts100.1  (5)
HLM150.5
  • 1-a Represents the one plasmid CYP2C19 expression construct.