Comparison of Clint between commercial enzyme sources and E. coli membranes expressing CYPs
| Probe Reaction | Isoform | Clint | |
|---|---|---|---|
| Commercial Source | E. coli Membranes | ||
| μl · min−1 · nmol−1 | |||
| EROD | CYP1A2 | 800 | 600 |
| Naproxen O-demethylation | CYP2C9 | 40 | 20 |
| Diazepam N-demethylation | CYP2C19 | 60 | 120 |
| Dextromethorphan O-demethylation | CYP2D6 | 340 | 200 |
| ErythromycinN-demethylation | CYP3A4 | 40 | 140 |
Clint = Vmax/Km, calculated from the values in Table 3. Commercial sources of CYPs were B-lymphoblasts expressing CYP1A2, 2C9, 2D6, and 3A4, and baculosomes expressing CYP2C19.