Table 1

Estimates of apparent V_max and K_m of HL for CYP probe drugs

CYP	Substrate	Concentration	Microsomal Preparations	K_m	V_max
		μM		μM	nmol/min/mg
CYP1A2	phenacetin	25–150	MHL-G^1-b	48.7 ± 11.0^1-a	2.2 ± 0.3
			HL-7	67.1 ± 15.0	0.4 ± 0.2
			HL-14	52.1 ± 25.4	2.5 ± 1.3
CYP2C9	tolbutamide	25–200	HL-29	152.1 ± 31.5	0.03 ± 0.01
			MHL-B^1-c	163.5 ± 26.7	0.26 ± 0.04
CYP2C19	omeprazole	25–150	HL-29	42.2 ± 12.9	0.13 ± 0.04
			MHL-B^1-c	55.1 ± 9.2	0.11 ± 0.01
CYP2D6	dextromethorphan	10–75	MHL-D^1-d	26.2 ± 7.5	0.06 ± 0.01
			HL-29	26.9 ± 5.5	0.34 ± 0.03
			HL-10	22.3 ± 4.0	0.20 ± 0.02
CYP3A	dextromethorphan	100–750	HL-10	236.3 ± 54.9	0.34 ± 0.08
			MHL-B^1-c	320.8 ± 75.4	0.84 ± 0.14

↵1-a Each value indicates the mean ± S.D. of three to eight different determinations.
↵1-b Mixed HL-5, -9, -10.
↵1-c Mixed HL-15, -19, -20.
↵1-d Mixed HL-8, -11, -14.