Table 3

Effect of murine in vivo inducers/depressors on COH activity in human and mouse hepatocytes

TreatmentTimeHuman HepatocytesMurine Hepatocytes
ConcentrationActivityConcentrationActivity
h % of control % of control
Phenobarbital481 mM84  ± 71.5 mM3-b 380  ± 303-c
2 mM3-a 104  ± 11
721 mM223  ± 173-c ND3-d
Rifampicin4850 μM3-b 82  ± 1810 μM3-a 320  ± 143-c
7225 μM114  ± 11ND
Pyrazole4810 mM3-a 760  ± 463-c 10 mM3-a 460  ± 613-c
Cobalt chloride48100 μM3-a 90  ± 2210 μM2 100  ± 75
200 μM 63
72100 μM188  ± 63-c ND
200 μM182  ± 283-c
Griseofulvin48100 μM99  ± 24100 μM604  ± 1643-c
200 μM3-a 141  ± 25
72100 μM156  ± 32ND
Aminotriazole48200 μM3-b 117  ± 13100 μM3-a 100  ± 35
2 mM3-a 171
72200 μM3-b 182  ± 133-c
Thioacetamide4850 μM3-b  8210 μM3-a 70  ± 89
Clofibric acid48500 μM3-a 85  ± 12500 μM3-a 80  ± 51
  • 3-a  Concentrations close to the highest nontoxic concentration of the compound.

  • 3-b  Concentrations below the highest nontoxic concentration of the compound.

  • 3-c  P < .05.

  • 3-d  ND, not determined.