Table 1

CYP isoform characterization of individual HLM pools

IsoformProbeBatch 1Batch 2Batch 3
pmol/min/mg
P450 contentCO difference spectrum3201-a 2801-a 4301-a
P450 reductaseCytochrome creduction690008400075000
CYP7-EthoxycoumarinO-demethylation255210240
CYP1A2Phenacetin O-dethylation366310193
CYP2A6Coumarin 7-hydroxylation172008190058600
CYP2E1Chlorzoxazone 6-hydroxyline101019111441
CYP2CMephenytoin 4-hydroxylation2723311
CYP2D6DextromethorphanO-demethylation20084106
CYP3ATestosterone 6β-hydroxylation260015001900
CYP4ALauric acid 11-hydroxylation100016001600
  • 1-a  Picomoles of CYP per milligram of microsomal protein.