Microsome Specimen | 4-NP-Gluc Formation1-a | NNAL-Gluc Formation1-b | NNAL-Gluc II:NNAL- Gluc I1-c |
---|---|---|---|
μmol/mg | nmol/mg | ||
Liver no. 22 | ND1-d | 2.6 | 3.5 |
Liver no. 109 | ND | 0.6 | 2.8 |
Liver no. 61297 | 3.1 | 1.0 | 3.8 |
Lung no. 17682 | 0.015 | Not detected | |
Lung no. 17702 | 0.024 | Not detected | |
Lung no. SEM | 0.004 | Not detected | |
Lung no. 99604 | 0.006 | Not detected | |
RLM (PB1)1-e | 2.8 | 0.7 | 0.2 |
↵1-a 4-NP glucuronidation assays were performed for 2 h using microsomal protein (50 μg) from rat liver, human liver, and human lung tissues.
↵1-b NNAL glucuronidation assays were performed for 2 h using microsomal protein from rat liver (50 μg, 40 min incubation), human liver (0.1 mg, 40 min incubation), and human lung (0.1–2.5 mg, 2 h incubation) tissues.
↵1-c Ratios of NNAL-Gluc II to NNAL-Gluc I in glucuronidation assays using microsomal protein from rat liver, human liver, and human lung tissues.
↵1-d ND, not done.
↵1-e RLM, rat liver microsomes from a phenobarbitol-treated rat, PB1 (see Ren et al., 1999).