Table 1

Assay parameters

SubstrateBzResBFCBQDBF
Molecular weight303320235512
Apparent K mor S50 (μM)30 ± 7>16044 ± 1 0.87 ± 0.12
ApparentV max 0.12 ± 0.01Not applicable17 ± 1.922 ± 1
Substrate concentration (μM)5050401
Enzyme concentration (nM)151101
Incubation time (min)30203010

Vmax is expressed as nmol product/min/nmol CYP3A4.