Apparent Km, Vmax, and intrinsic clearance values of the CYP2D6-mediated O-dealkylation of MAMC and the 4-substituted 7-methoxycoumarins
| Substrate | Km Value | VmaxValue | Vmax/Km |
|---|---|---|---|
| μM | min−1 | min−1/μM | |
| MAMC | 11.52 ± 1.32 | 2.81 ± 0.14 | 0.244 ± 0.039 |
| MMAMC | 5.09 ± 0.94 | 1.72 ± 0.12 | 0.338 ± 0.084 |
| EMAMC | 1.00 ± 0.08 | 1.41 ± 0.07 | 1.410 ± 0.183 |
| PMAMC | 0.20 ± 0.03 | 0.91 ± 0.03 | 4.550 ± 0.819 |
| BMAMC | 0.08 ± 0.02 | 0.70 ± 0.03 | 8.750 ± 2.537 |
| DiMMAMC | 10.56 ± 0.36 | 1.20 ± 0.02 | 0.114 ± 0.005 |
| MMPyrC | 16.31 ± 0.73 | 1.15 ± 0.02 | 0.071 ± 0.004 |
CYP2D6 was expressed in a human lymphoblastoid cell line. Values are given as the mean ± S.D. (n = 3).