Table 2

List of oligonucleotide probes generated for analysis of CYP expression by bDNA signal amplification

Probe ID	GenBank ID	Target^2-a	Function^2-b	Probe Sequence^2-c	Specificity
CYP1A1
CYP1A1.2001	I00732	570–591	CE	gtattcagcctctttgctcacgTTTTTctcttggaaagaaagt	CYP1A1
CYP1A1.2032	I00732	658–679	LE	agatgacattggccactgacacTTTTTaggcataggacccgtgtct	CYP1A1
CYP1A1.2033	I00732	680–699	LE	tctgccaaagcatatggcacTTTTTaggcataggacccgtgtct	CYP1A1
CYP1A1.2080	I00732	700–721	BL	cttggtcatcgtggtcataacg	CYP1A1
CYP1A1.2003	I00732	722–745	CE	ttagattgactatgctgagcagctTTTTTctcttggaaagaaagt	CYP1A1
CYP1A1.2034	I00732	746–766	LE	taacctccccaaactcattgcTTTTTaggcataggacccgtgtct	CYP1A1
CYP1A1.2035	I00732	767–787	LE	cagctgggtatccagaaccagTTTTTaggcataggacccgtgtct	CYP1A1
CYP1A1.2081	I00732	788–811	BL	ggtaacggaggataggaatgaagt	CYP1A1
CYP1A1.2082	I00732	812–833	BL	gcatccagggaagagttaggga	CYP1A1
CYP1A1.2083	I00732	834–860	BL	tagaacttcttattcaagtccttgaag	CYP1A1
CYP1A1.2004	I00732	861–885	CE	tttgattagcttcttcatgaaactgTTTTTctcttggaaagaaagt	CYP1A1
CYP1A1.2084	I00732	887–910	BL	ccttctcaaatgtcctgtagtgct	CYP1A1
CYP1A1.2036	I00732	912–931	LE	ctgtgatgtcccggatgtggTTTTTaggcataggacccgtgtct	CYP1A1
CYP1A1.2037	I00732	932–953	LE	tgacaatgctcaatgaggctgtTTTTTaggcataggacccgtgtct	CYP1A1
CYP1A1.2005	I00732	954–972	CE	ctcgtccagcctcctgtccTTTTTctcttggaaagaaagt	CYP1A1
CYP1A2
CYP1A2.2038	X01031	501–522	LE	ctgtttcaaatccagctccaaaTTTTTaggcataggacccgtgtct	CYP1A2
CYP1A2.2006	X01031	569–591	CE	catgaatcttcctctgcaccttgTTTTTctcttggaaagaaagt	CYP1A2
CYP1A2.2039	X01031	592–612	LE	caatcaccgtgtccagctcctTTTTTaggcataggacccgtgtct	CYP1A2
CYP1A2.2007	X01031	613–631	CE	cgtggctgccgatctctgcTTTTTctcttggaaagaaagt	CYP1A2
CYP1A2.2088	X01031	632–651	BL	gctggggtctgtcagaaagc	CYP1A2
CYP1A2.2008	X01031	652–671	CE	gaaggcctccagatatggcaTTTTTctcttggaaagaaagt	CYP1A2
CYP1A2.2009	X01031	672–695	CE	tgtgtatcggtagatctccaggatTTTTTctcttggaaagaaagt	CYP1A2
CYP1A2.2089	X01031	696–716	BL	gatggtgaaggggacaaagga	CYP1A2
CYP1A2.2040	X01031	717–734	LE	cctcgttgtgctgtggggTTTTTaggcataggacccgtgtct	CYP1A2
CYP1A2.2010	X01031	757–776	CE	gcagcactccttgggaatgtTTTTTctcttggaaagaaagt	CYP1A2
CYP1A2.2091	X01031	777–799	BL	acctgccactggtttatgaagat	CYP1A2
CYP1A2.2041	X01031	800–821	LE	ccactgcttctcatcatggttgTTTTTaggcataggacccgtgtct	CYP1A2
CYP1A2.2092	X01031	822–841	BL	cggaacacaaaggggtcttt	CYP1A2
CYP1A2.2093	X01031	842–861	BL	tggtaagaaaccgctctggg	CYP1A2
CYP1A2.2042	X01031	862–880	LE	tcgatggccgtgttgtcatTTTTTaggcataggacccgtgtct	CYP1A2
CYP1A2.2094	X01031	881–903	BL	tcaccttctcactcagggtcttg	CYP1A2
CYP1A2.2095	X01031	904–921	BL	ttcccaagccgaagagca	CYP1A2
CYP1A2.2096	X01031	922–937	BL	ccaatgcaccggcgct	CYP1A2
CYP1A2.2011	X01031	938–955	CE	cacttggccgggatctccTTTTTctcttggaaagaaagt	CYP1A2
CYP1A2.2012	X01031	956–978	CE	tggctaagaagaggaagacttccTTTTTctcttggaaagaaagt	CYP1A2
CYP1A2.2013	X01031	979–998	CE	ctccagctgatgcaggaggaTTTTTctcttggaaagaaagt	CYP1A2
CYP2B1/2
CYP2B.2044	J00719	566–584	LE	tccaacaggcgcaggaactTTTTTaggcataggacccgtgtct	CYP2B1/2
CYP2B.2099	J00719	585–606	BL	ggaaaaggtccggtagaacagc	CYP2B1/2
CYP2B.2014	J00719	607–629	CE	tggctggagaatgaacttaggagTTTTTctcttggaaagaaagt	CYP2B1/2
CYP2B.2100	J00719	630–652	BL	acccagagaagaactcaaacacc	CYP2B1/2
CYP2B.2101	J00719	653–675	BL	ggcaccaggaaagtatttcagga	CYP2B1/2
CYP2B.2102	J00719	676–699	BL	gaggtttttggagatttgtctgtg	CYP2B1/2
CYP2B.2015	J00719	700–722	CE	ccaatgtaatcgaggatttcctgTTTTTctcttggaaagaaagt	CYP2B1/2
CYP2B.2045	J00719	723–744	LE	cctgtgcttctccacaatatggTTTTTaggcataggacccgtgtct	CYP2B1/2
CYP2B.2016	J00719	745–763	CE	cgcttgggtctaaggtggcTTTTTctcttggaaagaaagt	CYP2B1/2
CYP2B.2103	J00719	764–785	BL	gtgtcgatgaagtctcgtggag	CYP2B1/2
CYP2B.2104	J00719	786–806	BL	ttctccatgcgcagaaggtaa	CYP2B1/2
CYP2B.2046	J00719	807–828	LE	tgtgtggtggttcgacttctccTTTTTaggcataggacccgtgtct	CYP2B1/2
CYP2B.2017	J00719	829–852	CE	catgaggttctcatgatggaactcTTTTTctcttggaaagaaagt	CYP2B1/2
CYP2B.2018	J00719	853–876	CE	aaagaagagagagagcagggagatTTTTTctcttggaaagaaagt	CYP2B1/2
CYP2B.2105	J00719	877–895	BL	tgctggtctcagtgccagc	CYP2B1
CYP2B.2107	J00719	896–915	BL	accatagcggagtgtggtgc	CYP2B1/2
CYP2B.2108	J00719	916–937	BL	ggtacttgagcatcagcaggaa	CYP2B1/2
CYP2B.2047	J00719	938–958	LE	ggactttctctgcgacatgggTTTTTaggcataggacccgtgtct	CYP2B1/2
CYP2B.2048	J00719	959–982	LE	cgatcacctgatcaatctccttttTTTTTaggcataggacccgtgtct	CYP2B1/2/3/12/15
CYP2E1
CYP2E1.2049	S48325	501–525	LE	ctccatctctatgaggagacagtcaTTTTTaggcataggacccgtgtct	CYP2E1
CYP2E1.2019	S48325	526–548	CE	ggttcttggctgtgtttttccttTTTTTctcttggaaagaaagt	CYP2E1
CYP2E1.2109	S48325	549–574	BL	cagaaacattttccattgtgtacatg	CYP2E1
CYP2E1.2110	S48325	575–594	BL	gaacaggtcggccaaagtca	CYP2E1
CYP2E1.2106	S48325	598–616	BL	tggtggtctcagttcctgc	CYP2E1
CYP2E1.2050	S48325	617–639	LE	gagcccatatctcagagttgtgcTTTTTaggcataggacccgtgtct	CYP2E1
CYP2E1.2051	S48325	640–663	LE	ttctgggtatttcatgaggatcagTTTTTaggcataggacccgtgtct	CYP2E1
CYP2E1.2111	S48325	664–690	BL	aatttcttcatgaagtttctcttcaat	CYP2E1
CYP2E1.2112	S48325	691–710	BL	cttggcccaataaccctgtc	CYP2E1
CYP2E1.2052	S48325	711–727	LE	tgacagcagggacgcggTTTTTaggcataggacccgtgtct	CYP2E1
CYP2E1.2053	S48325	728–751	LE	tgtagggcatatccagtctgtctcTTTTTaggcataggacccgtgtct	CYP2E1
CYP2E1.2054	S48325	752–771	LE	ctcatgcaccacagcatccaTTTTTaggcataggacccgtgtct	CYP2E1
CYP2E1.2113	S48325	772–796	BL	ggacaagattgatgaatctctggat	CYP2E1
CYP2E1.2020	S48325	797–818	CE	gcttcatggggtaggttggaagTTTTTctcttggaaagaaagt	CYP2E1
CYP2E1.2114	S48325	819–842	BL	ccttggaacacagtatctctggtt	CYP2E1
CYP2E1.2115	S48325	843–865	BL	ctgtacccttggggatgacatat	CYP2E1
CYP2E1.2116	S48325	866–888	BL	ggagtccagagttggaatcacaa	CYP2E1
CYP2E1.2117	S48325	889–914	BL	ggaaactcatggctgtcatataagag	CYP2E1
CYP2E1.2021	S48325	915–940	CE	gctcaggtttaaacttctctggatctTTTTTctcttggaaagaaagt	CYP2E1
CYP2E1.2022	S48325	941–963	CE	cttcccattttcattcaggaaatTTTTTctcttggaaagaaagt	CYP2E1

Probe ID	GenBank ID	Target^2-a	Function^2-b	Probe Sequence^2-c	Specificity
CYP3A1/23
CYP3A.2059	D13912	616–637	LE	ccaaatgatgtgctggtgatcaTTTTTaggcataggacccgtgtct	CYP3A1/2/23
CYP3A.2060	D13912	638–661	LE	ttgttgagggaatcaacattcactTTTTTaggcataggacccgtgtct	CYP3A1/23
CYP3A.2061	D13912	662–681	LE	ccacaaaaggatccttcgggTTTTTaggcataggacccgtgtct	CYP3A1/23
CYP3A.2023	D13912	683–706	CE	attcttaagagcttcttggctttcTTTTTctcttggaaagaaagt	CYP3A1/23
CYP3A.2120	D13912	707–729	BL	agaacaacggatcaaaaaaatca	CY3A1/2/23
CYP3A.2121	D13912	730–755	BL	gaggaatggaaagagtactactgaca	CYP3A1/2/23
CYP3A.2062	D13912	756–780	LE	tgtttaacatctcatatactggcgtTTTTTaggcataggacccgtgtct	CYP3A1/23
CYP3A.2122	D13912	781–802	BL	gaatcttttgggaacatgcaga	CYP3A1/23
CYP3A.2123	D13912	804–831	BL	tgtacacaaatttttgaaaaattctat	CYP3A1/23
CYP3A.2024	D13912	832–851	CE	caggcgggtttccttcattcTTTTTctcttggaaagaaagt	CYP3A1/23
CYP3A.2063	D13912	852–873	LE	ctcgatgcttctgcacagaatcTTTTTaggcataggacccgtgtct	CYP3A1/23
CYP3A.2124	D13912	874–896	BL	catcatcagctgaagaaaatcca	CYP3A1/23
CYP3A.2064	D13912	897–923	LE	tttgtctttagaatcattatgagcattTTTTTaggcataggacccgtgtct	CYP3A1/23
CYP3A.2025	D13912	924–946	CE	tcggatagggctgtatgagattcTTTTTctcttggaaagaaagt	CYP3A1/23
CYP3A.2026	D13912	947–968	CE	tgactgggctgtgatctccataTTTTTctcttggaaagaaagt	CYP3A1/23
CYP3A.2065	D13912	970–994	LE	tcatatccagcaaaaataaaaatgaTTTTTaggcataggacccgtgtct	CYP3A1/23
CYP4A2/3
CYP4A.2027	M57719	501–526	CE	tctcccatttgtctagcattatattgTTTTTctcttggaaagaaagt	CYP4A3
CYP4A.2066	M57719	527–548	LE	gggtgatcctggtcatcaagctTTTTTaggcataggacccgtgtct	CYP4A2/3
CYP4A.2125	M57719	549–575	BL	aaggagacatagtggaagatctctaga	CYP4A2/3
CYP4A.2126	M57719	576–601	BL	acttcataacagtgtccagtgtcatc	CYP4A2/3
CYP4A.2028	M57719	602–621	CE	gccctgatggctgaaagcacTTTTTctcttggaaagaaagt	CYP4A3
CYP4A.2127	M57719	622–646	BL	tggaatttacatccaactgaacact	CYP4A3
CYP4A.2128	M57719	647–667	BL	cgacagccttggtgtaggacc	CYP4A3
CYP4A.2129	M57719	669–694	BL	gaaagaaagtcaggttgtttagatcc	CYP4A3
CYP4A.2067	M57719	695–716	LE	ccataaaaggcactcctcacacTTTTTaggcataggacccgtgtct	CYP4A2/3
CYP4A.2068	M57719	717–742	LE	aggacatattgtagatgatgctgttcTTTTTaggcataggacccgtgtct	CYP4A2/3
CYP4A.2069	M57719	743–761	LE	cgggacaaacggccatcagTTTTTaggcataggacccgtgtct	CYP4A2/3
CYP4A.2130	M57719	762–778	BL	caatctggcaggcccgg	CYP4A2/3
CYP4A.2070	M57719	779–801	LE	cactccatctgtgtgctcatgagTTTTTaggcataggacccgtgtct	CYP4A3
CYP4A.2029	M57719	802–823	CE	gctgagccttcctcattttgatTTTTTctcttggaaagaaagt	CYP4A2/3
CYP4A.2030	M57719	824–845	CE	tgaagctcttcctcattctgcaTTTTTctcttggaaagaaagt	CYP4A2/3
CYP4A.2031	M57719	846–865	CE	gcctcttcttcctggccttcTTTTTctcttggaaagaaagt	CYP4A2/3

↵2-a Target relates to the target sequence of the mRNA transcript and the numbers signify the first nucleotide of the target sequence and extend through the last nucleotide of the target sequence.
↵2-b Function relates to the function of the oligonucleotide probe in the bDNA assay (CE, capture probe; LE, label probe; BL, blocker probe).
↵2-c Probe sequence relates to the oligonucleotide probe sequence that includes a poly T spacer with or without a CE or LE extension. All oligonucleotide probe sequences are the reverse complement of the respective mRNA target sequence.