Table 1

Chrysin glucuronidation by recombinant UGT1A isoforms

UGT IsoformKmVmaxVmax/Km
μM pmol/min/mg protein ml/min/mg protein
UGT1A10.353601.02
UGT1A612.81570.012
UGT1A91.712100.71
UGT1A4N.D.N.D.N.D.

Apparent enzyme kinetic parameters were obtained by fitting the data, as in Fig. 3, to the Michaelis-Menten equation. Mean values from two experiments are shown.

  • N.D., activity not detectable.