Nonspecific binding of Rh123 to collagen-coated polystyrene and Permanox culture dishes
| % of Initial Dose | Polystyrene | Permanox | ||
|---|---|---|---|---|
| HBSS | Ca2+-free HBSS | HBSS | Ca2+-free HBSS | |
| Fourth rinse solution | 0.34 ± 0.02 | 0.76 ± 0.02 | N.D. | N.D. |
| Triton X-100 | 2.34 ± 0.04 | 4.89 ± 0.13 | 0.08 ± 0.02 | 0.14 ± 0.01 |
Culture dishes were treated according to the described procedure for determination of Rh123 accumulation in SC rat hepatocytes (dishes were incubated for 30 min with 1 μM Rh123 in standard or Ca2+-free HBSS and subsequently rinsed four times with ice-cold standard HBSS; dishes were then incubated with 0.5% Triton X-100 to recover all residual Rh123). Values represent average (±S.E.M., n= 6) amounts of Rh123 in fourth rinsing solutions and in Triton X-100 solutions, expressed as percentage of the amount of Rh123 added at the start of the incubation.
N.D., not detectable.