Table 3

Comparison of biliary clearance of Rh123 and digoxin (1 μM) in SC rat hepatocytes (in vitro Clbile) with literature values of biliary clearance measured in vivo in rats and/or in IPRL

SubstrateIn Vitro ClbilePredicted3-aClBinIn Vivo ClBinIPRL ClBin
ml/min/kg
Rh1238.0  ± 0.819.9  ± 5.83-b 18.3  ± 1.83-c 12.3  ± 1.23-d
Digoxin4.9  ± 0.8313.5  ± 5.83-b 14.7  ± 3.73-e
  • 3-a  Predicted ClBin(intrinsic biliary clearance) was calculated according to the linear relationship between intrinsic in vivo biliary clearance and the in vitro biliary clearance in SC rat hepatocytes for taurocholate, [d-pen2,5]enkephalin, methotrexate, salicylate, and inulin as established previously (in vivo ClBin = 2.04 × in vitro Clbile + 3.52; Liu et al., 1999b).

  • 3-b  Standard deviation on estimation of predicted values from linear relationship between in vitro and in vivo biliary clearance.

  • 3-c  Intrinsic biliary clearance of unchanged Rh123 in vivo in rats (Sweatman et al., 1990) calculated as described under Experimental Procedures.

  • 3-d  Intrinsic biliary clearance of unchanged Rh123 in rat IPRL (Jager et al., 1997) calculated as described under Experimental Procedures.

  • 3-e  Intrinsic biliary clearance of unchanged digoxin in rat IPRL (Hedman and Meijer, 1998) calculated as described under Experimental Procedures.