Apparent kinetic parameters for the N-glucuronidation of 1-substituted imidazole substrates by human UGT1A4 expressed in baculovirus-infected insect cells
| 1-Substituted Imidazoles | Km | Vmax | Vmax/ Km |
|---|---|---|---|
| mM | nmol/min/mg | μl/min/mg | |
| 1-Phenyl ( 1a ) | 3.15 ± 0.32 | 0.33 ± 0.01 | 0.11 ± 0.01 |
| 1-Benzyl ( 1b ) | 2.48 ± 0.44 | 0.35 ± 0.03 | 0.14 ± 0.02 |
| 1-(4-Methoxyphenyl) ( 1d ) | 0.70 ± 0.06 | 0.31 ± 0.02 | 0.45 ± 0.01 |
| 1-(4-Chlorophenyl) ( 1e ) | 0.67 ± 0.05 | 0.29 ± 0.01 | 0.43 ± 0.02 |
| 1-(4-Bromophenyl) ( 1f ) | 0.18 ± 0.02 | 0.17 ± 0.01 | 0.95 ± 0.14 |
| 1-(4-Trifluoromethyl) ( 1g ) | 0.32 ± 0.01 | 0.16 ± 0.01 | 0.50 ± 0.03 |
Data are given as mean ± S.D. of three determinations, each carried out in duplicate.