Recovery of testosterone 6β-hydroxylase activity after an 18-h dialysis following a 20-min preincubation with DDB and NADPH in human liver microsomes
| DDB | % of Control Activity Remaining | ||
|---|---|---|---|
| +NADPH −Dialysis | +NADPH+Dialysis | −NADPH +Dialysis | |
| μM | |||
| 0 | 100 | 100 | 100 |
| 0.25 | 67.6.1 ± 8.7 | 90.1 ± 9.0 | 94.2 ± 3.9 |
| 0.5 | 47.2 ± 8.3 | 83.0 ± 6.9 | 101.0 ± 14.1 |
| 1 | 40.9 ± 5.0 | 61.2 ± 8.0 | 89.3 ± 6.0 |
| 5 | 14.8 ± 1.0 | 23.4 ± 1.6 | 87.3 ± 10.5 |
The uninhibited rates of the reaction in column 1, 2, and 3 were 0.53, 0.47, and 0.52 nmol/min/mg of protein, respectively. Each value represents the mean ± S.D. of three determinations.