Table 1

Experimental conditions and Michaelis-Menten kinetics for different rCYP fluorescence assays

CYP	Enzyme Amount	Incubation Time	Substrate	Excitation Wavelength	Emission Wavelength	Enzyme Kinetic Parameters
CYP	Enzyme Amount	Incubation Time	Substrate	Excitation Wavelength	Emission Wavelength	K_m	V_max
	pmol	min		nm		μM	nmol/min/nmol CYP
1A2	1 [0.5–2]	20 [10–35]	CEC	405	460	3	14
2C9	3 [0.5–3]	50 [10–60]	MFC	405	535	47	0.4
2C19	5 [0.5–10]	40 [10–45]	MFC	405	535	75	0.4
2D6	2 [0.5–2]	35 [10–45]	MAMC	390	460	100	132
3A4	2 [0.5–2]	30 [10–30]	BFC	405	535	13	1.8

Values in brackets represent linearity range.

CEC, 3-cyano-7-ethoxycoumarin; MAMC, 7-methoxy-4-(aminomethyl)-coumarin; BFC, 7-benzyloxy-4-trifluoromethylcoumarin.