Table 1

Experimental conditions and Michaelis-Menten kinetics for different rCYP fluorescence assays

CYPEnzyme AmountIncubation TimeSubstrateExcitation WavelengthEmission WavelengthEnzyme Kinetic Parameters
KmVmax
pmol min nm μM nmol/min/nmol CYP
1A21 [0.5–2]20 [10–35]CEC405460314
2C93 [0.5–3]50 [10–60]MFC405535470.4
2C195 [0.5–10]40 [10–45]MFC405535750.4
2D62 [0.5–2]35 [10–45]MAMC390460100132
3A42 [0.5–2]30 [10–30]BFC405535131.8

Values in brackets represent linearity range.

  • CEC, 3-cyano-7-ethoxycoumarin; MAMC, 7-methoxy-4-(aminomethyl)-coumarin; BFC, 7-benzyloxy-4-trifluoromethylcoumarin.