Table 2

Reproducibility of kinetic parameters from microsomes prepared from beagle dogs

SubstrateVmaxKmCLint
DLM 1DLM 2DLM 3Mean ± S.D.DLM 1DLM 2DLM 3Mean ± S.D.DLM 1DLM 2DLM 3Mean ± S.D.
nmol/min/mg μM μl/min/mg
Gemfibrozil5.612.39.49.1  ± 3.4200360310290  ± 8229353031  ± 3.2
Androstanediol12.35.64.27.4  ± 4.3430270220310  ± 11028201922  ± 4.9
Ketoprofen2.94.52.33.2  ± 1.11100150018001500  ± 3502.831.32.4  ± 0.9
Furosemide0.91.00.70.9  ± 0.2130097011001100  ± 1700.610.60.7  ± 0.2

DLM 1 were prepared from the liver of a single beagle dog (used to produce the data in Table 1). DLM 2 and 3 were each prepared from two sets of pooled DLM (each prepared from five separate dog livers). Data are from individual expts.