β-Glucuronidase activity in tissue microsomal samples from human and dog
| Tissue | β-Glucuronidase Activity −Saccharic Acid 1,4-Lactone | β-Glucuronidase Activity +Saccharic Acid 1,4-Lactone | Inhibition |
|---|---|---|---|
| pmol/min/mg | % | ||
| HLM | 58 ± 8.5 | 10 ± 5.2 | 83 |
| HKM | 35 ± 9.0 | 22 ± 17 | 37 |
| DLM | 36 ± 7.9 | 7.6 ± 7.2 | 79 |
| DKM | 130 ± 21 | 27 ± 34 | 79 |
HLM were produced by pooling microsomes prepared from six human livers, each one characterized as shown in Fig. 1. HKM were prepared from the kidney of a single human. DLM were prepared from the liver of a single beagle dog. DKM were prepared from the kidney of a single beagle dog. Data are mean ± S.D. of three individual expts. performed in duplicate.