Table 2

Summary of P450 activities measured in the NCI cell line panel

Cell LineP450 Reductase7-ECODCoumarin7-EROD7-BROD
Part A
 Leukemia
   1 K-562 + + + + + + + +
   2 MOLT-4 + + + + + + +
   3 CCRF-CEM + + + (+) +
   4 RPMI-8226 + + + +
   5 HL-60(TB) + + (+)
   6 SR + + + + + + + +
 CNS
   7 SF-268 + + (+) +
   8 SF-295 + + + + (+)N.A. + + +
   9 SF-539 + + + + + +
  10 SNB-19 + + + + + + + +
  11 SNB-75 + + (+) + + + + +
  12 U261 + + (+) + + +
 Breast
  13 BT-549 + + + + N.A. + + +
  14 HS-578T + + + + + + + + + +
  15 MCF7 + + + (+)(+) + + + + +
  16 MCF7/ADR-RES + + + (+) + +
  17 MDA-MB-231/ATCC + + + + + + + +
  18 MDA-MB-435 + + + + + + + + + +
  19 MDA-N + + + + + + + + +
  20 T-47D + + + + + (+) +
 Colon
  21 COLO 205 + + + N.A. +
  22 HCC-2998 + + + + (+) + + + + + +
  23 HCT-116 + + + + + + + + +
  24 HCT-15 + + + + + + + + + + +
  25 HT29 + + + + (+) + + +
  26 KM12 + + + + + + + + + + + + + + +
  27 SW-620 + + + + + + + + + +
 Lung
  28 A549/ATCC + + + + + + + + + + + + +
  29 EKVX + + + + (+) + + + + + +
  30 HOP-62 + + + + + + +
  31 HOP-92 + + (+) + + +
  32 NCI-H322M + + + + + + + + +
  33 NCI-H226 + + + N.A. + +
  34 NCI-H23 + + + + + (+) + + + + + + +
  35 NCI-H460 + + + + + + + + + + + +
  36 NCI-H522 + + + + + + + (+) + +
 Melanoma
  37 LOX-IMVI + + + +
  38 M14 + + + + + +
  39 MALME-3M + + + + + + + +
  40 SK-MEL-2 + + + + + + + + + + + + + + +
  41 SK-MEL-28 + + + + + + + + +
  42 SK-MEL-5 + + + + + + + + + + + + + +
  43 UACC-257 + + + + + + + + + + + +
  44 UACC-62 + + + + + (+) + + + + + +
 Ovarian
  45 IGORV1 + + + + + + + + + + + + +
  46 OVCAR-3 + + + + + + (+) + + + +
  47 OVCAR-4 + + + + + + + + + + + + + + + + + + + + +
  48 OVCAR-5 + N.A. + +
  49 OVCAR-8 + + + + + +
  50 SK-OV-3 + + + +
 Prostate
  51 DU-145 + + + + + + + +
  52 PC-3 + + + + + + +
 Renal
  53 786-0 + + + + + + +
  54 A498 + + + + + + + + + + + + +
  55 ACHN + + + + + +
  56 CAKI-1 + + + + + + + +
  57 RXF-393 + + + (+) +
  58 SN12C + + + + + +
  59 TK-10 + + + + + (+) + + +
  60 UO-31 + + + + + N.A. + +
nmol/min/mg pmol/min/mg
Part B. Definition of Scales Used in Part A
+ 5–400.2–0.40.1–0.20.2–14–20
+ + 40–750.4–0.750.2–0.31–2.520–30
+ + + 75–1250.75–1.250.3–0.52.5–430–40
+ + + + 125–2501.25–2.00.5–0.74–540–50
+ + + + + >250>2.0>0.7>5>50

Higher activities are compared on a scale of “+” to “+++++” as defined in Part B below. Numbers shown for each cell line are used in the figures to identify each cell line.

  • N.A., not assayed.

  • −, activity not readily detectable; (+), activity at limit of detection.